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Colorimetry

Determine the concentration of a colored substance using absorbance measurements

Step 1: Prepare Standards (Serial Dilution)

CuSO₄

Stock Solution
0.10 M

1
2
3
4
5

Cuvettes 1–5 correspond to 0.02, 0.04, 0.06, 0.08, and 0.10 mol dm⁻³.

Calibration Curve

Absorbance vs. concentration (A = 15 × c)

0.000.020.040.060.080.100.120.00.51.01.52.0Concentration (mol dm⁻³)Absorbance

Beer-Lambert Law

  • 0.02 M → A = 0.30; 0.10 M → A = 1.50
  • After all 5 standards are measured, the line of best fit passes through the origin.